Degradation of the Indospicine Toxin from Indigofera spicata by a Mixed Population of Rumen Bacteria

The leguminous plant species, Indigofera linnaei and spicata are distributed throughout the rangeland regions of Australia compound indospicine (L-2-amino-6-amidinohexanoic acid) found in these palatable forage plants acts as a hepatotoxin can accumulate meat ruminant livestock wild camels. In this study, bovine rumen fluid was cultivated an vitro fermentation system provided with material ability resulting mixed microbial populations to degrade determined using UPLC–MS/MS over 14 day time period. were 16S rRNA gene amplicon sequencing showed distinct, time-related changes occurring rumen-derived microbes adapted conditions nutritional substrates by material. Within eight days commencement, completely degraded within fermenter, forming degradation products 2-aminopimelamic acid 2-aminopimelic 24 h approach enabled development specifically adapted, population which has potential be used drench for reducing toxic side-effects toxin accumulation associated ingestion grazing livestock.